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1 article(s) from Clardy, Jon

Bacterial terpene biosynthesis: challenges and opportunities for pathway engineering

Eric J. N. Helfrich,
Geng-Min Lin,
Christopher A. Voigt and
Jon Clardy

Beilstein J. Org. Chem. 2019, 15, 2889–2906, doi:10.3762/bjoc.15.283

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Figure 1: Examples of bioactive terpenoids.

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Figure 2: Repetitive electrophilic and nucleophilic functionalities in terpene and type II PKS-derived polyke...

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Figure 3: Abundance and distribution of bacterial terpene biosynthetic gene clusters as determined by genome ...

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Figure 4: Terpenoid biosynthesis. Terpenoid biosynthesis is divided into two phases, 1) terpene scaffold gene...

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Figure 5: Mechanisms for type I, type II, and type II/type I tandem terpene cyclases. a) Tail-to-head class I...

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Figure 6: Functional TC characterization. a) Different terpenes were produced when hedycaryol (18) synthase a...

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Figure 7: Selected examples of terpene modification by bacterial CYPs. a) Hydroxylation [89]. b) Carboxylation, h...

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Figure 8: Off-target effects observed during heterologous expression of terpenoid BGCs. Unexpected oxidation ...

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Figure 9: TC promiscuity and engineering. a) Spata-13,17-diene (39) synthase (SpS) can take C₁₅ and C₂₅ oligo...

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Figure 10: Substrate promiscuity and engineering of CYPs. a) Selected examples from using a CYP library to oxi...

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Figure 11: Engineering of terpenoid pathways. a) Metabolic network of terpenoid biosynthesis. Toxic intermedia...

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Published 29 Nov 2019

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